A troponin switch that regulates muscle contraction by stretch instead of calcium

被引:69
作者
Agianian, B
Krzic, U
Qiu, F
Linke, WA
Leonard, K
Bullard, B
机构
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
[2] Heidelberg Univ, Inst Physiol & Pathophysiol, Heidelberg, Germany
关键词
Ca2+ regulation; insect flight muscle; stretch activation; troponin C;
D O I
10.1038/sj.emboj.7600097
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The flight muscles of many insects have a form of regulation enabling them to contract at high frequencies. The muscles are activated by periodic stretches at low Ca2+ levels. The same muscles also give isometric contractions in response to higher Ca2+. We show that the two activities are controlled by different isoforms of TnC (F1 and F2) within single myofibrils. F1 binds one Ca2+ with high affinity in the C-terminal domain and F2 binds one Ca2+ in the C-terminal domain and one exchangeable Ca2+ in the N-terminal domain. We have characterised the isoforms and determined their effect on the development of stretch-activated and Ca2+-activated tension by replacing endogenous TnC in Lethocerus flight muscle fibres with recombinant isoforms. Fibres with F1 gave stretch-activated tension and minimal isometric tension; those with F2 gave Ca2+-dependent isometric tension and minimal stretch-activated tension. Regulation by a TnC responding to stretch rather than Ca2+ is unprecedented and has resulted in the ability of insect flight muscle to perform oscillatory work at low Ca2+ concentrations, a property to which a large number of flying insects owe their evolutionary success.
引用
收藏
页码:772 / 779
页数:8
相关论文
共 52 条
[1]   EFFECTS OF FIBER LENGTH AND CALCIUM-ION CONCENTRATION ON DYNAMIC-RESPONSE OF GLYCEROL EXTRACTED INSECT FIBRILLAR MUSCLE [J].
ABBOTT, RH .
JOURNAL OF PHYSIOLOGY-LONDON, 1973, 231 (02) :195-208
[2]   A novel method of extraction of TnC from skeletal muscle myofibrils [J].
Allhouse, LD ;
Potter, JD ;
Ashley, CC .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1999, 437 (05) :695-701
[3]  
BERS DM, 1994, METHOD CELL BIOL, V40, P3
[5]   TROPONIN OF ASYNCHRONOUS FLIGHT-MUSCLE [J].
BULLARD, B ;
LEONARD, K ;
LARKINS, A ;
BUTCHER, G ;
KARLIK, C ;
FYRBERG, E .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (03) :621-637
[6]   ARTHRIN - A NEW ACTIN-LIKE PROTEIN IN INSECT FLIGHT-MUSCLE [J].
BULLARD, B ;
BELL, J ;
CRAIG, R .
JOURNAL OF MOLECULAR BIOLOGY, 1985, 182 (03) :443-454
[7]   ACTIN MEDIATED REGULATION OF MUSCLE-CONTRACTION [J].
CHALOVICH, JM .
PHARMACOLOGY & THERAPEUTICS, 1992, 55 (02) :95-148
[8]   Phosphorylation-dependent power output of transgenic flies: An integrated study [J].
Dickinson, MH ;
Hyatt, CJ ;
Lehmann, FO ;
Moore, JR ;
Reedy, MC ;
Simcox, A ;
Tohtong, R ;
Vigoreaux, JO ;
Yamashita, H ;
Maughan, DW .
BIOPHYSICAL JOURNAL, 1997, 73 (06) :3122-3134
[9]   HOW DO BEES SHIVER [J].
ESCH, H ;
GOLLER, F ;
HEINRICH, B .
NATURWISSENSCHAFTEN, 1991, 78 (07) :325-328
[10]  
FARAH CS, 1994, J BIOL CHEM, V269, P5230