Somatostatin as a regulator of first-trimester human trophoblast functions

被引：4

作者：

Biondi, C. ^{[1
]}

Ferretti, M. E. ^{[1
]}

Lunghi, L. ^{[1
]}

Medici, S. ^{[1
]}

Cervellati, F. ^{[1
]}

Abelli, L. ^{[2
]}

Bertoni, F. ^{[2
]}

Adinolfi, E. ^{[3
]}

Vesce, F. ^{[4
]}

Bartolini, G. ^{[5
]}

Papi, A. ^{[5
]}

D'Andrea, S. ^{[6
]}

Berton, S. ^{[6
]}

Baldassarre, G. ^{[6
]}

机构：

[1] Univ Ferrara, Sect Gen Physiol, Dept Biol & Evolut, I-44100 Ferrara, Italy

[2] Univ Ferrara, Sect Comparat Anat, Dept Biol & Evolut, I-44100 Ferrara, Italy

[3] Univ Ferrara, Sect Gen Pathol, Dept Expt & Diagnost Med, I-44100 Ferrara, Italy

[4] Univ Ferrara, Sect Obstet & Gynecol, Dept Biomed Sci & Adv Therapy, I-44100 Ferrara, Italy

[5] Univ Bologna, Dept Evolut Expt Biol, Bologna, Italy

[6] CRO, Div Expt Oncol 2, Aviano, Italy

来源：

PLACENTA | 2008年 / 29卷 / 08期

关键词：

trophoblast-derived cell lines; somatostatin; cyclic AMP; phospholipase A2; cell proliferation migration;

D O I：

10.1016/j.placenta.2008.05.009

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

We have tested the hypothesis that human early trophoblast is a target for somatostatin (SRIF) regulatory actions. We report for the first time that SSTR2A and 2B transcripts and proteins are present in first-trimester human chorionic villi and the trophoblast-derived HTR-8/SVneo and JAR cells. In both cell lines, SSTR are functional since SRIF inhibits cyclic AMP pathway, Stimulates arachidonic acid release and enhances cell proliferation. Moreover. in HTR-8/SVneo cells, considered a good model of first-trimester EVT SRIF also enhances migration. An involvement of the cyclic AMP pathway in mediating SRIF effects on proliferation and migration is suggested. Our data support the idea that SRIF regulates early trophoblast functions mainly through an interaction with SSTR2. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：660 / 670

页数：11

共 38 条

[1]

Immunohistochemical and ultrastructural evidence of adrenal chromaffin cell subtypes in sea bass Dicentrarchus labrax (L) [J].

Abelli, L ;

Gallo, VP ;

Civinini, A ;

Mastrolia, L .

GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1996, 102 (01) :113-122

[2]

Somatostatin receptor-mediated arachidonic acid mobilization: evidence for partial agonism of synthetic peptides [J].

Alderton, F ;

Fan, ZPD ;

Humphrey, PPA .

BRITISH JOURNAL OF PHARMACOLOGY, 2001, 132 (03) :760-766

[3]

Prostaglandin E2 inhibits proliferation and migration of HTR-8/SVneo cells, a human trophoblast-derived cell line [J].

Biondi, C. ;

Ferretti, M. E. ;

Pavan, B. ;

Lunghi, L. ;

Gravina, B. ;

Nicoloso, M. S. ;

Vesce, F. ;

Baldassarre, G. .

PLACENTA, 2006, 27 (6-7) :592-601

[4]

BITO H, 1994, J BIOL CHEM, V269, P12722

[5]

BROWN BL, 1972, ADV CYCL NUCL RES<D>, V2, P25

[6]

Expression of somatostatin receptor SST4 in human placenta and absence of octreotide effect on human placental growth hormone concentration during pregnancy [J].

Caron, P ;

Buscail, L ;

Beckers, A ;

Esteve, JP ;

Igout, A ;

Hennen, G ;

Susini, C .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (11) :3771-3776

[7]

Somatostatin (SRIF) modulates distinct signaling pathways in rat pituitary tumor cells; negative coupling of SRIF receptor subtypes 1 and 2 to arachidonic acid release [J].

Cervia, D ;

Fiorini, S ;

Pavan, B ;

Biondi, C ;

Bagnoli, P .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2002, 365 (03) :200-209

[8]

Differential efficacies of somatostatin receptor agonists for G-protein activation and desensitization of somatostatin receptor subtype 4-mediated responses [J].

Engström, M ;

Savola, JM ;

Wurster, S .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2006, 316 (03) :1262-1268

[9]

17β-estradiol stimulates arachidonate release from human amnion-like WISH cells through a rapid mechanism involving a membrane receptor [J].

Fiorini, S ;

Ferretti, ME ;

Biondi, C ;

Pavan, B ;

Lunghi, L ;

Paganetto, G ;

Abelli, L .

ENDOCRINOLOGY, 2003, 144 (08) :3359-3367

[10]

ESTABLISHMENT AND CHARACTERIZATION OF 1ST TRIMESTER HUMAN TROPHOBLAST CELLS WITH EXTENDED LIFE-SPAN [J].

GRAHAM, CH ;

HAWLEY, TS ;

HAWLEY, RG ;

MACDOUGALL, JR ;

KERBEL, RS ;

KHOO, N ;

LALA, PK .

EXPERIMENTAL CELL RESEARCH, 1993, 206 (02) :204-211

← 1 2 3 4 →