Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana

被引：70

作者：

Oguchi, K ^{[1
]}

Liu, HT ^{[1
]}

Tamura, K ^{[1
]}

Takahashi, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

FEBS LETTERS | 1999年 / 457卷 / 03期

关键词：

telomerase; telomere; reverse transcriptase; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(99)01083-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The AtTERT protein contains the conserved reverse transcriptase motifs 1, 2 and A-E aswell as the TERT-specific T motif, Reverse transcription-polymerase chain reaction analysis and an assay of telomerase activity revealed that both AtTERT mRNA and telomerase activity are abundant in shoot apical meristems but are not detectable in rosette leaves. (C) 1999 Federation of European Biochemical Societies.

引用

页码：465 / 469

页数：5

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