Increased interleukin-6 messenger RNA expression in macrophage-cocultured endometrial stromal cells in adenomyosis

被引：50

作者：

Yang, JH ^{[1
]}

Wu, MY ^{[1
]}

Chang, DY ^{[1
]}

Chang, CH ^{[1
]}

Yang, YS ^{[1
]}

Ho, HN ^{[1
]}

机构：

[1] Natl Taiwan Univ Hosp, Dept Obstet & Gynecol, Taipei 100, Taiwan

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2006年 / 55卷 / 03期

关键词：

adenomyosis; endometrial stromal cell; interleukin-6; macrophage;

D O I：

10.1111/j.1600-0897.2005.00363.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Problem To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis. Method of study Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr. Results Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis. Conclusion IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.

引用

页码：181 / 187

页数：7

共 32 条

[1]

Akoum A, 1996, HUM REPROD, V11, P2269

[2] Interleukin-8 in the human endometrium [J].

Arici, A ;

Seli, E ;

Senturk, LM ;

Gutierrez, LS ;

Oral, E ;

Taylor, HS .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (05) :1783-1787

[3]

BRAUN DP, 1994, FERTIL STERIL, V61, P78

[4] Relationship between apoptosis and the number of macrophages in eutopic endometrium from women with and without endometriosis [J].

Braun, DP ;

Ding, J ;

Shen, J ;

Rana, N ;

Fernandez, BB ;

Dmowski, WP .

FERTILITY AND STERILITY, 2002, 78 (04) :830-835

[5] Cytolysis of eutopic and ectopic endometrial cells by peripheral blood monocytes and peritoneal macrophages in women with endometriosis [J].

Braun, DP ;

Gebel, H ;

Rana, N ;

Dmowski, WP .

FERTILITY AND STERILITY, 1998, 69 (06) :1103-1108

[6] Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy [J].

Chen, HF ;

Chao, KH ;

Shew, JY ;

Yang, YS ;

Ho, HN .

HUMAN REPRODUCTION, 2004, 19 (07) :1647-1654

[7] Defective production of interleukin-11 by decidua and chorionic villi in human anembryonic pregnancy [J].

Chen, HF ;

Lin, CY ;

Chao, KH ;

Wu, MY ;

Yang, YS ;

Ho, HN .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2002, 87 (05) :2320-2328

[8]

Chen JJW, 2003, CLIN CANCER RES, V9, P729

[9] Disruption of the endometrial-myometrial border during pregnancy as a risk factor for adenomyosis [J].

Curtis, KM ;

Hillis, SD ;

Marchbanks, PA ;

Peterson, HB .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2002, 187 (03) :543-544

[10] Decreased apoptosis and sensitivity to macrophage mediated cytolysis of endometrial cells in endometriosis [J].

Dmowski, WP ;

Gebel, H ;

Braun, DP .

HUMAN REPRODUCTION UPDATE, 1998, 4 (05) :696-701

← 1 2 3 4 →