Enzymatic hydrolysis of polysaccharide-rich particulate organic waste

被引:18
作者
He, PJ [1 ]
Lü, F
Shao, LM
Pan, XJ
Lee, DJ
机构
[1] Tongji Univ, State Key Lab Pollut Control & Reclamat, Shanghai 200092, Peoples R China
[2] Natl Taiwan Univ, Dept Chem Engn, Taipei 10764, Taiwan
关键词
pH value; acetate; enzymatic hydrolysis; inhibition; particulate organic waste; kinetic models;
D O I
10.1002/bit.20834
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Hydrolysis of organic particulates in rapid fermentative processes can be inhibited. The volatile fatty acids (VFA) released during fermentation reduce pH. Whether VFA or the drop in pH inhibits hydrolysis is unclear. The effects of pH and acetate on the enzymatic hydrolysis of a potato sample that contains both carbohydrate and protein were studied at fixed pH (5-9) in the presence/absence of 20 g/L of acetate. Experimental results showed that the effects of pH and acetate on the hydrolysis of carbohydrate differed from those on the hydrolysis of protein. Numerous kinetic models fitted the hydrolysis data obtained during the first 40 h of hydrolysis when inhibitory effects were insignificant. The Chen-Hashimoto model was used herein to fit the hydrolysis data obtained during 144 h of reaction. Also, the non-competitive inhibition model of three inhibitors (H+, OH-, total/undissociated/dissociated acetate) successfully described the inhibition of the hydrolysis of both carbohydrate and protein. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码:1145 / 1151
页数:7
相关论文
共 39 条
[1]   DEGRADATION OF VOLATILE FATTY-ACIDS BY DIFFERENTLY ENRICHED METHANOGENIC CULTURES - KINETICS AND INHIBITION [J].
AGUILAR, A ;
CASAS, C ;
LEMA, JM .
WATER RESEARCH, 1995, 29 (02) :505-509
[2]  
ANDREWS JF, 1971, ADV CHEM SER, P126
[3]  
Angelidaki I, 1999, BIOTECHNOL BIOENG, V63, P363, DOI 10.1002/(SICI)1097-0290(19990505)63:3<363::AID-BIT13>3.0.CO
[4]  
2-Z
[5]  
[Anonymous], TRIB CEBEDEAU
[6]   Effect of acid speciation on solid waste liquefaction in an anaerobic acid digester [J].
Babel, S ;
Fukushi, K ;
Sitanrassamee, B .
WATER RESEARCH, 2004, 38 (09) :2417-2423
[7]   EFFECT OF PROPIONATE TOXICITY ON METHANOGEN-ENRICHED SLUDGE, METHANOBREVIBACTER-SMITHII, AND METHANOSPIRILLUM-HUNGATII AT DIFFERENT PH VALUES [J].
BARREDO, MS ;
EVISON, LM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (06) :1764-1769
[8]  
Batstone D. J, 2002, ANAEROBIC DIGESTION
[9]   A NEW APPROACH TO THE KINETIC-STUDY OF ANAEROBIC DEGRADATION OF THE ORGANIC FRACTION OF MUNICIPAL SOLID-WASTE [J].
CECCHI, F ;
ALVAREZ, JM ;
TRAVERSO, PG ;
MEDICI, F ;
FAZZINI, G .
BIOMASS, 1990, 23 (02) :79-102
[10]   SUBSTRATE UTILIZATION KINETIC-MODEL FOR BIOLOGICAL TREATMENT PROCESSES [J].
CHEN, YR ;
HASHIMOTO, AG .
BIOTECHNOLOGY AND BIOENGINEERING, 1980, 22 (10) :2081-2095