Development and application of proteomics technologies in Saccharomyces cerevisiae

被引：33

作者：

Kolkman, A

Slijper, M

Heck, AJR

机构：

[1] Univ Utrecht, Dept Biomol Mass Spectrometry, Bijvoet Ctr, NL-3584 CA Utrecht, Netherlands

[2] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3584 CA Utrecht, Netherlands

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 12期

关键词：

D O I：

10.1016/j.tibtech.2005.09.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Proteomics research focuses on the identification and quantification of 'all' proteins present in cells, organisms or tissue. Proteomics is technically complicated because it encompasses the characterization and functional analysis of all proteins that are expressed by a genome. Moreover, because the expression levels of proteins strongly depend on complex regulatory systems, the proteome is highly dynamic. This review focuses on the two major proteomics methodologies, one based on 2D gel electrophoresis and the other based on liquid chromatography coupled to mass spectrometry. The recent developments of these methodologies and their application to quantitative proteomics are described. The model system Saccharomyces cerevisiae is considered to be the optimal vehicle for proteomics and we review studies investigating yeast adaptation to changes in (nutritional) environment.

引用

页码：598 / 604

页数：7

共 73 条

[1] A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard [J].