Rapid determination of caffeine in tea leaves

被引：42

作者：

Horie, H ^{[1
]}

Nesumi, A ^{[1
]}

Ujihara, T ^{[1
]}

Kohata, K ^{[1
]}

机构：

[1] Natl Inst Vegetable & Tea Sci, Shizuoka 4288501, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 942卷 / 1-2期

关键词：

tea leaves; caffeine; polyphenols;

D O I：

10.1016/S0021-9673(01)01360-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

For the purpose of efficient screening of low-caffeine tea shoots, a method for the rapid determination of caffeine was developed using high-performance liquid chromatography. Polyvinylpolypyrrolidone was packed in a pre-column and used to remove polyphenols from tea extracts on-line. The concentrations of caffeine extracted from powdered tea leaves at 50degreesC during 1 day could be analyzed in 2-5 min intervals. The pre-column and the analytical column could be used for the analysis of more than 2000 samples. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：271 / 273

页数：3

共 4 条

[1]

Analysis of tea components by high-performance liquid chromatography and high-performance capillary electrophoresis [J].