Inflammatory markers in skeletal muscle of older adults

Older adults have an increase in circulating markers of inflammation. The current study examined whether there is an increase in the expression of inflammatory markers within the vastus lateralis, a major locomotive muscle, of older adults, and if so, whether the reduction in muscle strength and aerobic capacity in older adults is related to increased muscle inflammation. Skeletal muscle biopsies were taken from older adults (n = 17, 67 +/- A 1.6 years) and young individuals (n = 16, 24 +/- A 0.6 years) under resting and fasting conditions. Muscle was analyzed for mRNA levels of intracellular inflammatory molecules (MCP1, TNF alpha and IL-1 beta) and total cellular protein abundance of cytokines, chemokines and kinases (IL-6, IL-8, MCP1, TNF alpha, p65 (NF-kappa B), JNK1/2 and STAT3). MCP1 expression was significantly higher (p < 0.05; 50 %, mRNA and 40 %, protein) in elderly than younger participants, as was IL-8 (4 %). No detectable difference in kinase protein expression was observed for STAT3, JNK or p65 (NF-kappa B), TNF alpha or IL-6. Muscle strength was lower in the elderly compared to the young group (1.55 +/- A 0.17 vs. 2.56 +/- A 0.13 Nm/kg, p < 0.001). The elderly group also had a significantly lower VO2peak compared to the young group (24.9 +/- A 1.9 vs. 39.3 +/- A 1.9, p < 0.001), but muscle strength and VO2peak were not correlated with the examined inflammatory markers. Older adults have increased MCP1 (mRNA and protein abundance) and IL-8 (protein abundance) and also reduced muscle strength and VO2peak. However, the reduction in muscle strength and VO2peak was not related to the increase in muscle inflammatory markers in this cohort.