Adherence of recombinant pneumococcal surface adhesin A (rPsaA)-coated particles to human nasopharyngeal epithelial cells for the evaluation of anti-PsaA functional antibodies

被引：24

作者：

Romero-Steiner, S ^{[1
]}

Caba, J ^{[1
]}

Rajam, G ^{[1
]}

Langley, T ^{[1
]}

Floyd, A ^{[1
]}

Johnson, SE ^{[1
]}

Sampson, JS ^{[1
]}

Carlone, GM ^{[1
]}

Ades, E ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Res Dis Immunol Sect, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA

来源：

VACCINE | 2006年 / 24卷 / 16期

关键词：

pneumococcal surface adhesin A; adherence; functional antibodies;

D O I：

10.1016/j.vaccine.2006.01.042

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pneumococcal surface adhesin A (PsaA) is a pneumococcal vaccine candidate. In this study, we detect functional antibodies to PsaA by using carboxylate-modified fluospheres coated with either recombinant non-lipidated PsaA (rPsaA) or synthetic peptides with relevant epitopes of PsaA. Peptides P1-P3 were derived from phage display sequences; peptides P4-P7 were homologous to rPsaA. P1- and P4-coated fluospheres had similar adherence to Detroit 562 nasopharyngeal cells when compared to rPsaA-coated fluospheres. Homologous and heterologous competitive inhibitions with peptides in solution determined the specificity of the adherence. There was no significant difference (P = 0.25) between the inhibition of adherence of rPsaA- and P4-coated fluospheres. This study indicates that P1 and P4 contain a functional epitope(s) for the adherence of PsaA to nasopharyngeal cells making them suitable targets for the measurement of functional antibodies to PsaA. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：3224 / 3231

页数：8

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