Cloning and characterization of rat leukotriene B4 receptor

被引：37

作者：

Toda, A

Yokomizo, T

Masuda, K

Nakao, A

Izumi, T

Shimizu, T

机构：

[1] Univ Tokyo, Dept Biochem & Mol Biol, Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Dept Nephrol & Endocrinol, Bunkyo Ku, Tokyo 1130033, Japan

[3] Univ Tokyo, Fac Med, Bunkyo Ku, Tokyo 1130033, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 262卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1006/bbrc.1999.1284

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leukotriene B-4 (LTB4) is a potent chemoattractant for neutrophils and eosinophils. cDNAs for LTB4 receptor (BLT) have been cloned from human, mouse, and guinea pig. Here we report the isolation of BLT from rat genomic library. Rat BLT consists of 351 amino acids with homologies of 80.2, 93.2, and 71.6%, to human, mouse, and guinea pig BLT, respectively. When expressed in human embryonic kidney (HEK)-293 cells, rat BLT showed a specific and high-affinity binding to LTB4 with a Kd value of 0.68 nM (mean, n = 3). Northern blot analysis showed that BLT is exclusively expressed in polymorphonuclear leukocytes. Furthermore, the expression of BLT was high in proteosepeptone-activated peritoneal macrophages, while the resident macrophages did not show significant expression. The present results suggest important roles of LTB4 in macrophage recruitment and activation. (C) 1999 Academic Press.

引用

页码：806 / 812

页数：7

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