Evidence For an association of ndh B, ndh J gene products and ferredoxin-NADP-reductase as components of a chloroplastic NAD(P)H dehydrogenase complex

被引：108

作者：

Guedeney, G ^{[1
]}

Corneille, S ^{[1
]}

Cuine, S ^{[1
]}

Peltier, G ^{[1
]}

机构：

[1] CEA CADARACHE,DEPT ECOPHYSIOL VEGETALE & MICROBIOL,LAB ECOPHYSIOL PHOTOSYNTHESE,F-13108 ST PAUL DURANCE,FRANCE

来源：

FEBS LETTERS | 1996年 / 378卷 / 03期

关键词：

chlororespiration; cyclic electron flow; ferredoxin-NADP(+) reductase; NADH dehydrogenase; ndh gene; photosynthesis; Solanum tuberosum L;

D O I：

10.1016/0014-5793(95)01473-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using non-denaturing gel electrophoresis and staining with nitro-blue tetrazolium, me reveal the presence of two NAD(P)H oxidoreductase activity bands within thylakoids membranes of Solanum tuberosum L. Second dimension SDS-PAGE and Western analysis show that one of the activity bands contains several polypeptides, two of them being recognized by antibodies directed against peptides corresponding to conserved domains of chloroplastic genes products NDH B and NDH J (at 32 and 18 kDa, respectively). Both activity bands also contain a polypeptide (around 36 kDa) recognized by an antibody directed against ferredoxin-NADP(+)-reductase (FNR). We conclude from these results that both chloroplastic ndh B and ndh J gene products are components of a thylakoid NAD(P)H dehydrogenase complex. The association with FNR is suggested to allow the complex to use NADPH instead of NADH as a preferential substrate.

引用

页码：277 / 280

页数：4

共 29 条

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