COX-2 regulates E-cadherin expression through the NF-κB/Snail signaling pathway in gastric cancer

被引：108

作者：

Chen, Zhaofeng ^{[1
,2
]}

Liu, Min ^{[1
,2
]}

Liu, Xiaojun ^{[1
,2
]}

Huang, Shanshan ^{[3
]}

Li, Linlin ^{[1
]}

Song, Bo ^{[1
]}

Li, Hailong ^{[1
]}

Ren, Qian ^{[1
,2
]}

Hu, Zenan ^{[1
]}

Zhou, Yongning ^{[1
,2
]}

Qiao, Liang ^{[4
]}

机构：

[1] First Hosp Lanzhou Univ, Div Gastroenterol & Hepatol, Lanzhou 730000, Gansu, Peoples R China

[2] Lanzhou Univ, Key Lab Gastrointestinal Dis Gansu Prov, Lanzhou 730000, Gansu, Peoples R China

[3] Dongying Dist Hosp, Div Gastroenterol & Hepatol, Dongying, Shandong, Peoples R China

[4] Univ Sydney, Westmead Hosp, Westmead Millennium Inst, Western Clin Sch,Fac Med,Storr Liver Unit, Westmead, NSW 2145, Australia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2013年 / 32卷 / 01期

基金：

中国国家自然科学基金;

关键词：

gastric cancer; cyclooxygenase-2; nuclear factor-kappa B; Snail; E-cadherin; REPRESSOR SNAIL; INHIBITION; CELECOXIB; GROWTH; ZEB1;

D O I：

10.3892/ijmm.2013.1376

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Cyclooxygenase-2 (COX-2) participates in cancer invasion and metastasis by decreasing the expression of E-cadherin. However, the molecular mechanisms through which COX-2 regulates E-cadherin expression and function have not yet been fully elucidated. The aim of this study was to investigate the possible molecular mechanisms through which COX-2 regulates E-cadherin expression in gastric cancer. The mRNA and protein expression of COX-2, nuclear factor-kappa B (NF-kappa B), Snail and E-cadherin was detected in gastric cancer cells by quantitative PCR and western blot analysis, respectively. The expression of these genes was also detected in healthy gastric mucosa and gastric cancer tissues by immunohistochemistry. We detected various levels of COX-2, nuclear factor-kappa B (NF-kappa B), Snail and E-cadherin expression in the normal gastric mucosa and cancer tissues; however, the expression patterns differed: the increased expression of COX-2, NF-kappa B and Snail was observed in the gastric cancer tissues, whereas there was a considerable reduction in E-cadherin expression in the cancer tissues compared to the normal gastric mucosa. The expression patterns of COX-2, NF-kappa B and Snail were similar. The increased expression of COX-2 in the gastric cancer tissues closely correlated with the increased expression of NF-kappa B and Snail, but inversely correlated with the expression of E-cadherin. Treatment of the SGC7901 cells (which express high levels of COX-2) with celecoxib, a COX-2 inhibitor, not only led to a marked dose- and time-dependent decrease in the expression of COX-2, NF-kappa B and Snail, but also led to a significant increase in the expression of E-cadherin, and this was associated with a reduction in cell invasion. By contrast, the same treatment did not alter the expression of these genes in another gastric cancer cell line, MGC803 (which barely expresses COX-2). These data suggest that COX-2 regulates the expression of E-cadherin through the NF-kappa B and Snail signaling pathway in gastric cancer.

引用

页码：93 / 100

页数：8

共 26 条

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