An adenovirus-Epstein-Barr virus hybrid vector that stably transforms cultured cells with high efficiency

被引:38
作者
Tan, BT
Wu, L
Berk, AJ
机构
[1] Univ Calif Los Angeles, Inst Mol Biol, Dept Microbiol & Mol Genet, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Med, Dept Urol, Los Angeles, CA 90095 USA
关键词
D O I
10.1128/JVI.73.9.7582-7589.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
EBV episomes are nuclear plasmids that are stably maintained through multiple cell divisions in primate and canine cells (J. L. Yates, N. Warren, and B. Sugden, Nature 313:812-815, 1985). In this report, we describe the construction and characterization of an E1-deleted recombinant adenovirus vector system that delivers an EBV episome to infected cells. This adenovirus-EBV hybrid vector system utilizes Cre-mediated, site-specific recombination to excise an EBV episome from a target recombinant adenovirus genome. We demonstrate that this vector system efficiently delivers the EBV episome and stably transforms a large fraction of infected canine D-17 cells. Using a colony-forming assay, we demonstrate stable transformation of 37% of cells that survive the infection. However, maximal transformation efficiency is achieved at doses of the El-deleted recombinant adenoviruses that are toxic to the infected cells. Consequently, E1-deleted vector toxicity imposes a limitation on our current vector system.
引用
收藏
页码:7582 / 7589
页数:8
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