Cytokine gene expression during experimental Escherichia coli pyelonephritis in mice

被引:38
作者
Khalil, A
Brauner, A
Bakhiet, M
Burman, LG
Jaremko, G
Wretlind, B
Tullus, K
机构
[1] HUDDINGE HOSP,DEPT SURG,STOCKHOLM,SWEDEN
[2] KAROLINSKA HOSP,DEPT BACTERIOL,S-10401 STOCKHOLM,SWEDEN
[3] DANDERYD HOSP,SWEDISH INST INFECT DIS CONTROL,DEPT BACTERIOL,STOCKHOLM,SWEDEN
[4] HUDDINGE HOSP,DIV NEUROL,STOCKHOLM,SWEDEN
[5] KAROLINSKA HOSP,DEPT PATHOL,S-10401 STOCKHOLM,SWEDEN
[6] ST GORANS UNIV HOSP,DEPT PEDIAT,KAROLINSKA HOSP,KAROLINSKA INST,STOCKHOLM,SWEDEN
关键词
experimental acute pyelonephritis; obstruction; cytokines; interleukins; TGF-beta;
D O I
10.1016/S0022-5347(01)64282-4
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Purpose: We studied nine inflammatory and immunoregulatory cytokines in acute pyelonephritis and urethral obstruction in mice to better understand the processes underlying kidney inflammation and scarring. Materials and Methods: Experimental acute pyelonephritis was established in Bki NMRI outbred mice by bladder inoculation of Escherichia coli, followed by 6 h urethral obstruction. The numbers of cytokine mRNA expressing cells for interleukin-1 (IL-1), IL-4, IL-6, IL-10, IL-12, tumor necrosis factor alpha (TNF-alpha), TNF-beta, transforming growth factor beta (TGF-beta) and interferon gamma (IFN-gamma) were determined in the kidneys and spleens from the infected, non-infected but obstructed and untouched mice using in situ hybridization with radio-labelled oligonucleotide probes at 12 h, 48 h and 6 d after release of the urethral obstruction. Results: Kidney cell expression of IL-1, IL-6 and TNF-alpha mRNA was observed already at 12 h and persisted on day 6 in the infected animals. A significant proinflammatory cytokine response occurred also in the non-infected obstructed animals, albeit later and at lower levels. A marked increase of IL-4, IL-10, TGF-beta and IFN-gamma mRNA producing cells was also found in the kidneys of these two groups again with higher levels in the infected animals. Very high numbers of splenocytes expressing mRNA for IL-1 were observed especially in the infected animals. A high proportion of splenocytes further expressed mRNA for IL-6, TNF-alpha, IL-4, IL-10, IFN-gamma and TGF-beta, again with highest numbers in the infected group of animals. Conclusions: The present study extends previous knowledge about the local and systemic cytokine expression profiles during acute pyelonephritis and after urethral obstruction. Of particular interest was the marked kidney cell expression of mRNA for TGF-beta, presumed to be important both for obstructive and post-infectious renal scarring.
引用
收藏
页码:1576 / 1580
页数:5
相关论文
共 29 条
  • [1] BORDER WA, 1994, NEW ENGL J MED, V331, P1286
  • [2] SENSITIVE MESSENGER-RNA DETECTION USING UNFIXED TISSUE - COMBINED RADIOACTIVE AND NONRADIOACTIVE INSITU HYBRIDIZATION HISTOCHEMISTRY
    DAGERLIND, A
    FRIBERG, K
    BEAN, AJ
    HOKFELT, T
    [J]. HISTOCHEMISTRY, 1992, 98 (01) : 39 - 49
  • [3] HUMAN TRANSFORMING GROWTH FACTOR-BETA COMPLEMENTARY-DNA SEQUENCE AND EXPRESSION IN NORMAL AND TRANSFORMED-CELLS
    DERYNCK, R
    JARRETT, JA
    CHEN, EY
    EATON, DH
    BELL, JR
    ASSOIAN, RK
    ROBERTS, AB
    SPORN, MB
    GOEDDEL, DV
    [J]. NATURE, 1985, 316 (6030) : 701 - 705
  • [4] DIJKEMA R, 1986, METHOD ENZYMOL, V119, P441
  • [5] FASHENA SJ, 1980, J IMMUNOL, V145, P177
  • [6] INTERLEUKIN-10 REDUCES THE RELEASE OF TUMOR-NECROSIS-FACTOR AND PREVENTS LETHALITY IN EXPERIMENTAL ENDOTOXEMIA
    GERARD, C
    BRUYNS, C
    MARCHANT, A
    ABRAMOWICZ, D
    VANDENABEELE, P
    DELVAUX, A
    FIERS, W
    GOLDMAN, M
    VELU, T
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (02) : 547 - 550
  • [7] THE INFLAMMATORY RESPONSE AND TISSUE-DAMAGE - THE EXAMPLE OF RENAL SCARS FOLLOWING ACUTE RENAL INFECTION
    GLAUSER, MP
    MEYLAN, P
    BILLE, J
    [J]. PEDIATRIC NEPHROLOGY, 1987, 1 (04) : 615 - 622
  • [8] THE MURINE TUMOR NECROSIS FACTOR-BETA (LYMPHOTOXIN) GENE SEQUENCE
    GRAY, PW
    CHEN, E
    LI, CB
    TANG, WL
    RUDDLE, N
    [J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (09) : 3937 - 3937
  • [9] SUPPRESSION OF RENAL SCARRING BY PREDNISOLONE COMBINED WITH CIPROFLOXACIN IN ASCENDING PYELONEPHRITIS IN RATS
    HARAOKA, M
    MATSUMOTO, T
    TAKAHASHI, K
    KUBO, S
    TANAKA, M
    KUMAZAWA, J
    [J]. JOURNAL OF UROLOGY, 1994, 151 (04) : 1078 - 1080
  • [10] SHIGELLA INFECTION INDUCES CELLULAR ACTIVATION OF T-CELLS AND B-CELLS AND DISTINCT SPECIES-RELATED CHANGES IN PERIPHERAL-BLOOD LYMPHOCYTE SUBSETS DURING THE COURSE OF THE DISEASE
    ISLAM, D
    BARDHAN, PK
    LINDBERG, AA
    CHRISTENSSON, B
    [J]. INFECTION AND IMMUNITY, 1995, 63 (08) : 2941 - 2949