Mass spectrometry-based metabolomics of single yeast cells

被引：191

作者：

Ibanez, Alfredo J. ^{[1
]}

Fagerer, Stephan R. ^{[1
]}

Schmidt, Anna Mareike ^{[2
]}

Urban, Pawel L. ^{[4
]}

Jefimovs, Konstantins ^{[5
]}

Geiger, Philipp ^{[1
]}

Dechant, Reinhard ^{[3
]}

Heinemann, Matthias ^{[6
]}

Zenobi, Renato ^{[1
]}

机构：

[1] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

[2] ETH, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

[3] ETH, Inst Biochem, CH-8093 Zurich, Switzerland

[4] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 300, Taiwan

[5] Swiss Fed Labs Mat Sci & Technol EMPA, Lab Elect Metrol Reliabil, CH-8600 Dubendorf, Switzerland

[6] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 22期

关键词：

single-cell measurements; MALDI mass spectrometry; baker's yeast; GLYCOLYSIS; RESISTANCE; FLUX;

D O I：

10.1073/pnas.1209302110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (Delta PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.

引用

页码：8790 / 8794

页数：5

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