Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC-ESI MS/MS and their xanthine oxidase inhibition

被引：75

作者：

Kim, Dae Wook ^{[1
]}

Curtis-Long, Marcus J. ^{[2
]}

Yuk, Heung Joo ^{[1
]}

Wang, Yan ^{[1
]}

Song, Yeong Hun ^{[1
]}

Jeong, Seong Hun ^{[3
]}

Park, Ki Hun ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, IALS, Div Appl Life Sci, Plus Program BK21, Jinju 660701, South Korea

[2] Brandeis Univ, Grad Program Biochem & Biophys, Waltham, MA 02453 USA

[3] Namhae Garl Res Inst, Namhae 668812, South Korea

来源：

FOOD CHEMISTRY | 2014年 / 153卷

基金：

新加坡国家研究基金会;

关键词：

Angelica keiskei; MRM; Chalcone; Coumarin; Xanthine oxidase; PSORALEA-CORYLIFOLIA; MICE; FUROCOUMARINS; EXTRACTION; FLAVONOIDS; CHALCONES; COUMARINS; SAPONINS; ROOTS;

D O I：

10.1016/j.foodchem.2013.12.026

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Angelica keiskei is used as popular functional food stuff. However, quantitative analysis of this plant's metabolites has not yet been disclosed. The principal phenolic compounds (1-16) within A. keiskei were isolated, enabling us to quantify the metabolites within different parts of the plant. The specific quantification of metabolites (1-16) was accomplished by multiple reaction monitoring (MRM) using a quadruple tandem mass spectrometer. The limit of detection and limit of quantitation were calculated as 0.4-44 mu g/kg and 1.5-148 mu g/kg, respectively. Abundance and composition of these metabolites varied significantly across different parts of plant. For example, the abundance of chalcones (12-16) decreased as follows: root bark (10.51 mg/g) > stems (8.52 mg/g) > leaves (2.63 mg/g)> root cores (1.44 mg/g). The chalcones were found to be responsible for the xanthine oxidase (XO) inhibition shown by this plant. The most potent inhibitor, xanthoangelol inhibited XO with an IC50 of 8.5 mu M. Chalcones (12-16) exhibited mixed-type inhibition characteristics. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：20 / 27

页数：8

共 30 条

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