Potential of using real-time PCR-based detection of spoilage yeast in fruit juice - a preliminary study

被引：36

作者：

Casey, GD

Dobson, ADW ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland

[2] Natl Univ Ireland Univ Coll Cork, Natl Food Biotechnol Ctr, Cork, Ireland

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2004年 / 91卷 / 03期

关键词：

Candida krusei; real-time PCR;

D O I：

10.1016/j.ijfoodmicro.2003.09.002

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A real-time PCR system was used to differentiate between the common spoilage yeasts, Zygosaccharomyces bailii, 2 Zygosaccharomyces rouxii, Candida krusei, Rhodotorula glutinis and Saccharomyces cerevisiae, based on melting peak T. analysis of the 5.8S rDNA subunit and the adjacent ITS2 region of these yeasts. By using the real-time PCR system and by targeting the citrate synthase (cs 1) gene of C. krusei, it was possible to develop a sensitive detection system to both identify and quantitate the level of C. krusei growth in an artificially contaminated apple juice sample. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：327 / 335

页数：9

共 22 条

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[Anonymous], 1990, PCR PROTOCOLS, DOI DOI 10.1016/B978-0-12-372180-8.50042-1

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