XopR, a Type III Effector Secreted by Xanthomonas oryzae pv. oryzae, Suppresses Microbe-Associated Molecular Pattern-Triggered Immunity in Arabidopsis thaliana

被引:47
作者
Akimoto-Tomiyama, Chiharu [1 ]
Furutani, Ayako [1 ]
Tsuge, Seiji [2 ]
Washington, Erica J. [3 ]
Nishizawa, Yoko [4 ]
Minami, Eiichi [4 ]
Ochiai, Hirokazu [1 ]
机构
[1] Natl Inst Agrobiol Sci, Div Plant Sci, Plant Microbe Interact Res Unit, Tsukuba, Ibaraki 3058603, Japan
[2] Kyoto Prefectural Univ, Grad Sch Agr, Plant Pathol Lab, Kyoto 606, Japan
[3] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[4] Natl Inst Agrobiol Sci, Genetically Modified Organism Res Ctr, Dis Resistant Crops Res & Dev Unit, Tsukuba, Ibaraki 3058603, Japan
关键词
PLANT-PATHOGENIC BACTERIA; PSEUDOMONAS-SYRINGAE; INNATE IMMUNITY; FLAGELLIN PERCEPTION; DISEASE RESISTANCE; GENOME SEQUENCE; PLASMA-MEMBRANE; PROTEIN SECRETION; RECEPTOR; RICE;
D O I
10.1094/MPMI-06-11-0167
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Xanthomonas oryzae pv. oryzae is the causal agent of bacterial blight of rice. The XopR protein, secreted into plant cells through the type III secretion apparatus, is widely conserved in xanthomonads and is predicted to play important roles in bacterial pathogenicity. Here, we examined the function of XopR by constructing transgenic Arabidopsis thaliana plants expressing it under control of the dexamethasone (DEX)-inducible promoter. In the transgenic plants treated with DEX, slightly delayed growth and variegation on leaves were observed. Induction of four microbe-associated molecular pattern (MAMP)-specific early-defense genes by a nonpathogenic X. campestris pv. campestris hrcC deletion mutant were strongly suppressed in the XopR-expressing plants. XopR expression also reduced the deposition of callose, an immune response induced by flg22. When transiently expressed in Nicotiana benthamiana, a XopR::Citrine fusion gene product localized to the plasma membrane. The deletion of XopR in X. oryzae pv. oryzae resulted in reduced pathogenicity on host rice plants. Collectively, these results suggest that XopR inhibits basal defense responses in plants rapidly after MAMP recognition.
引用
收藏
页码:505 / 514
页数:10
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