QCAL - a novel standard for assessing instrument conditions for proteome analysis

被引：35

作者：

Eyers, Claire E. ^{[1
]}

Simpson, Deborah M. ^{[2
]}

Wong, Stephen C. C. ^{[1
]}

Beynon, Robert J. ^{[2
]}

Gaskell, Simon J. ^{[1
]}

机构：

[1] Univ Manchester, Manchester Interdisciplinary Bioctr, Michael Barber Ctr Mass Spectrometry, Sch Chem, Manchester M1 7DN, Lancs, England

[2] Univ Liverpool, Fac Vet Sci, Dept Vet Preclin Sci, Prot & Funct Genom Grp, Liverpool L69 3BX, Merseyside, England

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2008年 / 19卷 / 09期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/j.jasms.2008.05.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

If proteome datasets are to be collated, shared, and merged for higher level proteome analyses, there is a need for generally accepted strategies and reagents for optimization and standardization of instrument performance. At present, there is no single protein or peptide standard set that is capable of assessing instrument performance for peptide separation and analysis in this manner. To create such a standard, we have used the recently described QconCAT methodology to generate an artificial protein, QCAL. This protein, a concatenation of tryptic peptides that is expressed in E. coli, provides a stoichiometrically controlled mixture of peptides that are amenable to analysis by all commonly used instrumentation platforms for proteomics.

引用

页码：1275 / 1280

页数：6

共 19 条

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