In vivo microdialysis determination of collagen-induced serotonin release in rat blood

被引：22

作者：

Tsai, TH

Chen, CF

机构：

[1] NATL RES INST CHINESE MED,DEPT PHARMACOL,TAIPEI 11221,TAIWAN

[2] NATL YANG MING UNIV,DEPT PHARMACOL,TAIPEI 11221,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 730卷 / 1-2期

关键词：

microdialysis; serotonin; 5-hydroxyindoleacetic acid; collagen; epinephrine;

D O I：

10.1016/0021-9673(95)00977-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a sensitive microbore HPLC method coupled with an on-line microdialysis system to simultaneously measure endogenous 5-hydroxytryptamine (serotonin; 5-HT) and its major metabolite 5-hydroxyindoleacetic acid (5-HIAA) in the rat blood in vivo. A dialysis tube was placed in the right jugular vein. The validity of the procedure is demonstrated because analysis of the aggregating agents, collagen (1 mg/kg) plus epinephrine (0.3 mg/kg) after intravenous injection, showed that they induced an increase in 5-HT and 5-HIAA levels in the jugular vein of the rat.

引用

页码：121 / 123

页数：3

共 5 条

[1] PLATELET DENSE GRANULE RELEASE REACTION MONITORED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FLUOROMETRIC-DETERMINATION OF ENDOGENOUS SEROTONIN [J].