A putative new order of methanogenic Archaea inhabiting the human gut, as revealed by molecular analyses of the mcrA gene

被引：85

作者：

Mihajlovski, Agnes ^{[1
]}

Alric, Monique ^{[1
]}

Brugere, Jean-Francois ^{[1
]}

机构：

[1] Univ Clermont1, UFR Pharm, CRNH, IFR Sante Auvergne,ERT CIDAM, F-63001 Clermont Ferrand, France

来源：

RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 7-8期

关键词：

Methyl-coenzyme M reductase; Human intestinal microflora; Methanogens; Gut Microbiota;

D O I：

10.1016/j.resmic.2008.06.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The diversity of methanogenic Archaea from the gut of 6 humans was investigated by targeting mcrA, a molecular metabolic marker of methanogenesis. Three operational taxonomic units (OTUs) were recovered from about 400 clones analyzed, two of which were attributed to the expected Methanobacteriales Methanobrevibacter smithii (4 volunteers) and Methanosphaera stadtmanae (1 volunteer). The third OTU (1 Volunteer) corresponded to a distant phylotype that does not cluster with any of the five methanogenic orders. This result, also supported by 16S archaeal sequences retrieved from the same volunteer, strongly suggests there may be a sixth order and hence potential underestimation of the role of methanogens in gut physiology. (C) 2008 Elsevier Masson SAS. All rights reserved.

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页码：516 / 521

页数：6

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