Hypochlorite modification of high density lipoprotein: effects on cholesterol efflux from J774 macrophages

被引：35

作者：

Bergt, C ^{[1
]}

Reicher, H ^{[1
]}

Malle, E ^{[1
]}

Sattler, W ^{[1
]}

机构：

[1] Graz Univ Technol, Inst Med Biochem, A-8010 Graz, Austria

来源：

FEBS LETTERS | 1999年 / 452卷 / 03期

基金：

奥地利科学基金会;

关键词：

reverse cholesterol transport; high density lipoprotein modification; myeloperoxidase; hypochlorite;

D O I：

10.1016/S0014-5793(99)00677-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was aimed at investigating effects of hypochlorite (HOCl) modification of high density lipoproteins subclass 3 (HDL3) on their ability for cellular cholesterol removal from permanent J774 macrophages, Our findings indicate that HOCl (added as reagent or generated enzymatically by the myeloperoxidase/H2O2/Cl- system) damages apolipoprotein A-I, the major protein component of HDL3, Fatty acid analysis of native and HOCl-modified HDL3 revealed that unsaturated fatty acids in both major lipid subclasses (phospholipids and cholesteryl eaters) are targets for HOCl attack. HOCl modification resulted in impaired HDL3-mediated cholesterol efflux from J774 cells, regardless of whether reagent or enzymatically generated HOCl was used to modify the lipoprotein, Decreased cholesterol efflux was also observed after HOCl modification of reconstituted HDL particles, Impairment of cholesterol efflux from macrophages was noticed at low and physiologically occurring HOCl concentrations. (C) 1999 Federation of European Biochemical Societies.

引用

页码：295 / 300

页数：6

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