Simultaneous determination of ursodeoxycholic acid and its glycine-conjugate in serum as phenacyl esters using multidimensional liquid chromatography

被引：5

作者：

Choi, SJ

Jeong, CK

Lee, HM

Kim, K

Do, KS

Lee, HS ^{[1
]}

机构：

[1] Wonkwang Univ, Coll Pharm, Iksan 570749, South Korea

[2] Korea Res Inst Chem Technol, Toxicol Res Ctr, Taejon 305606, South Korea

[3] Taejeon Hlth & Environm Res Inst, Taejeon 305153, South Korea

来源：

CHROMATOGRAPHIA | 1999年 / 50卷 / 1-2期

关键词：

column liquid chromatography; norrow bore colums; column-switching; ursodeoxycholic acid; glycoursodeoxycholic acid;

D O I：

10.1007/BF02493624

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A narrowbore high-performance liquid chromatographic (HPLC) method using column switching is described for the simultaneous determination of ursodeoxycholic acid (UDCA) and glyco-UDCA (GUDCA) from serum samples as their phenacyl esters. Serum samples were subjected to a preliminary clean-up using octadecylsilane reversed-phase extraction and derivatized with phenacylbromide. The purification, fractionation and concentration of UDCA and GUDCA from the esterified serum sample were performed on-line by appropriate switching of columns. Limit of detection (LOD) of UDCA and GUDCA were 5 ng and the absolute mean recoveries averaged 84.4 +/- 8.2% and 85.2 +/- 8.4%, respectively. This method was successfully applied to the pharmacokinetic study of UDCA in rats and human.

引用

页码：96 / 100

页数：5

共 24 条

[1] URSODEOXYCHOLIC ACID IN THE TREATMENT OF CHOLESTEROL CHOLELITHIASIS .1. [J].