Gene analysis of trehalose-producing enzymes from hyperthermophilic archaea in Sulfolobales

被引:12
作者
Kobayashi, K [1 ]
Kato, M [1 ]
Miura, Y [1 ]
Kettoku, M [1 ]
Komeda, T [1 ]
Iwamatsu, A [1 ]
机构
[1] KIRIN BREWERY CO LTD, CENT LABS KEY TECHNOL, KANAZAWA KU, YOKOHAMA, KANAGAWA 236, JAPAN
关键词
trehalose; Sulfolobus; trehalose-producing enzyme; Southern analysis; genotype;
D O I
10.1271/bbb.60.1720
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genes encoding new trehalose-producing enzymes from S. acidocaldarius ATCC33909 were cloned to analyze the distribution of these genes in Sulfolobales. Comparison of the amino acid sequences with S, solfataricus KM1 showed approximately 50% similarity, Southern analysis suggests that homologues of the trehalose-producing enzyme genes exist widely in Sulfolobales and strains in Sulfolobales were classified into three kinds of genotypes.
引用
收藏
页码:1720 / 1723
页数:4
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