GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines

被引：20

作者：

Wang, YP ^{[1
]}

Sonntag, K

Rudloff, E

Wehling, P

Snowdon, RJ

机构：

[1] Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China

[2] Univ Giessen, Inst Plant Breeding & Crop Sci 1, D-35392 Giessen, Germany

[3] Inst Agr Crops, Fed Ctr Breeding Res Cultivated Plants, D-18190 Gross Lusewitz, Germany

来源：

PLANT CELL REPORTS | 2006年 / 25卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Brassica napus; Crambe abyssinica; addition lines; microspore culture; GISH;

D O I：

10.1007/s00299-005-0031-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two Brassica napus-Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F-2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding.

引用

页码：35 / 40

页数：6

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