Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori)

被引：32

作者：

Yang, Mingying ^{[1
]}

Shuai, Yajun ^{[1
]}

He, Wen ^{[1
]}

Min, Sijia ^{[1
]}

Zhu, Liangjun ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Anim Sci, Inst Appl Bioresource Res, Hangzhou 310058, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2012年 / 13卷 / 06期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

B. mori fibroin; silk gland; porous scaffolds; compressive strength; cell adhesion and growth activity; ALP activity; MESENCHYMAL STEM-CELLS; BIOMATERIALS;

D O I：

10.3390/ijms13067762

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In order to use a simple and ecofriendly method to prepare porous silk scaffolds, aqueous silk fibroin solution (ASF) was extracted from silk gland of 7-day-old fifth instar larvae of Bombyx mori (B. mori). SDS-page analysis indicated that the obtained fibroin had a molecular weight higher than 200 kDa. The fabrication of porous scaffolds from ASF was achieved by using the freeze-drying method. The pore of porous scaffolds is homogenous and tends to become smaller with an increase in the concentration of ASF. Conversely, the porosity is decreased. The porous scaffolds show impressive compressive strength which can be as high as 6.9 +/- 0.4 MPa. Furthermore, ASF has high cell adhesion and growth activity. It also exhibits high ALP activity. This implies that porous scaffolds prepared from ASF have biocompatibility. Therefore, the porous scaffolds prepared in this study have potential application in tissue engineering due to the impressive compressive strength and biocompatibility.

引用

页码：7762 / 7775

页数：14

共 39 条

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