Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin

被引：307

作者：

Teoh, KH ^{[1
]}

Polichuk, DR ^{[1
]}

Reed, DW ^{[1
]}

Nowak, G ^{[1
]}

Covello, PS ^{[1
]}

机构：

[1] Plant Biotechnol Inst, Saskatoon, SK S2N 0W9, Canada

来源：

FEBS LETTERS | 2006年 / 580卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

artemisinin; sesquiterpene; cytochrome P450; trichome; Artemisia annua;

D O I：

10.1016/j.febslet.2006.01.065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Artemisinin, a sesquiterpene lactone endoperoxide derived from the plant Artemisia annua, forms the basis of the most important treatments of malaria in use today. In an effort to elucidate the biosynthesis of artemisinin, an expressed sequence tag approach to identifying the relevant biosynthetic genes was undertaken using isolated glandular trichomes as a source of mRNA. A cDNA clone encoding a cytochrome P450 designated CYP71AV1 was characterized by expression in Saccharomyces cerevisiae and shown to catalyze the oxidation of the proposed biosynthetic intermediates amorpha4,11-diene, artemisinic alcohol and artemisinic aldehyde. The identification of the CYP71AV1 gene should allow for the engineering of semi-synthetic production of artemisinin in appropriate plant or microbial hosts. Crown Copyright (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies. All rights reserved.

引用

页码：1411 / 1416

页数：6

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