Application of tyramide signal amplification system to immunohistochemistry: A potent method to localize antigens that are not detectable by ordinary method

被引:98
作者
Toda, Y
Kono, K
Abiru, H
Kokuryo, K
Endo, M
Yaegashi, H
Fukumoto, M
机构
[1] Tohoku Univ, Inst Dev Aging & Canc, Dept Pathol, Aoba Ku, Sendai, Miyagi 9808575, Japan
[2] Kyoto Univ, Grad Sch Med, Ctr Human Remains, Kyoto 606, Japan
[3] Kyoto Univ, Grad Sch Med, Dept Neurosurg, Kyoto 606, Japan
关键词
antigen retrieval; archival sections; immunohistochemistry; tyramid signal amplification;
D O I
10.1046/j.1440-1827.1999.00875.x
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Tyramide signal amplification-avidin-biotin complex (TSA-ABC) method is a powerful technique used to detect antigens that are not detectable by ordinary immunohistochemistry. It is worth trying in cases where localization of antigens by the conventional method has failed and antibodies are precious.
引用
收藏
页码:479 / 483
页数:5
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