Transcriptome Analysis of Potato Tubers-Effects of Different Agricultural Practices

被引：34

作者：

van Dijk, Jeroen P. ^{[1
]}

Cankar, Katarina ^{[2
]}

Scheffer, Stanley J. ^{[1
]}

Beenen, Henriek G. ^{[1
]}

Shepherd, Louise V. T. ^{[3
]}

Stewart, Derek ^{[3
]}

Davies, Howard V. ^{[3
]}

Wilkockson, Steve J. ^{[4
]}

Lelfert, Carlo

Gruden, Kristina ^{[2
]}

Kok, Esther J. ^{[1
]}

机构：

[1] RIKILT Inst Food Safety, NL-6700 AE Wageningen, Netherlands

[2] Natl Inst Biol, Dept Biotechnol & Syst Biol, Ljubljana 1000, Slovenia

[3] Scottish Crop Res Inst, Plant Prod & Food Qual Programme, Dundee DD2 5DA, Scotland

[4] Univ Newcastle, Nafferton Ecol Farming Grp, Stocksfield NE43 7XD, Northd, England

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 04期

关键词：

Solanum tuberosum; cDNA microarray; gene expression; food safety; organic Input systems; GENE-EXPRESSION; PROTEINASE-INHIBITORS; ENZYME-INHIBITORS; MOLECULAR-CLONING; FAMILY; SAFETY; GLUCOSYLTRANSFERASE; IDENTIFICATION; HYBRIDIZATION; RESPONSES;

D O I：

10.1021/jf802815d

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptornics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments.

引用

页码：1612 / 1623

页数：12

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