The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

被引：33

作者：

Ferguson, GP ^{[1
]}

Chacko, AD ^{[1
]}

Lee, C ^{[1
]}

Booth, IR ^{[1
]}

机构：

[1] UNIV ABERDEEN,MARISCHAL COLL,DEPT MOLEC & CELL BIOL,ABERDEEN AB9 1AS,SCOTLAND

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 13期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/jb.178.13.3957-3961.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection. Lowering pHi further by incubation with acetate decreases the sensitivity of cells to MG.

引用

页码：3957 / 3961

页数：5

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