Cell refractive index tomography by digital holographic microscopy

被引:484
作者
Charrière, F [1 ]
Marian, A
Montfort, F
Kuehn, J
Colomb, T
Cuche, E
Marquet, P
Depeursinge, C
机构
[1] Ecole Polytech Fed Lausanne, Imaging & Appl Opt Inst, CH-1015 Lausanne, Switzerland
[2] Lyncee Tec SA, PSE A, CH-1005 Lausanne, Switzerland
[3] CHUV, Dept Psychiat, Ctr Neurosci Psychiat, CH-1008 Lausanne, Switzerland
关键词
D O I
10.1364/OL.31.000178
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated. (c) 2006 Optical Society of America.
引用
收藏
页码:178 / 180
页数:3
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