ERK MAP kinase is required in 1,25(OH)2D3-induced differentiation in human osteoblasts

被引：18

作者：

Chae, HJ

Jeong, BJ

Ha, MS

Lee, JK

Byun, JO

Jung, WY

Yun, YG

Lee, DG

Oh, S

Chae, SW

Kwak, YG

Kim, HH

Lee, ZH

Kim, HR ^{[1
]}

机构：

[1] Wonkwang Univ, Dept Dent Pharamcol, Sch Dent, Iksan 570749, Chonbuk, South Korea

[2] Wonkwang Univ, Wonkwang Dent Res Inst, Sch Dent, Iksan 570749, Chonbuk, South Korea

[3] Wonkwang Univ, Dept Maxillofacial Surg, Sch Dent, Iksan 570749, Chonbuk, South Korea

[4] Wonkwang Univ, Dept Oral Anat, Sch Dent, Iksan 570749, Chonbuk, South Korea

[5] Wonkwang Univ, Dept Oriental Prescript, Sch Dent, Iksan 570749, Chonbuk, South Korea

[6] Chosun Univ, Natl Res Lab Bone Metab, Kwangju 501759, South Korea

[7] Chosun Univ, Res Ctr Proteinous Mat, Kwangju 501759, South Korea

来源：

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | 2002年 / 24卷 / 01期

关键词：

D O I：

10.1081/IPH-120003401

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Expression of alkaline phosphatase(ALP)activity represents a key event during the differentiation processes of osteoblasts, and the level of ALP activity has been routinely used as a relative measure of differentiation stages of osteoblasts. In human osteoblasts, we showed that vitamin D-3 analogue, 1,25(OH)(2)D-3, had a stimulatory effect on ALP activity after 3 days, compared with control. The treatment of PD098059, an ERK MAP Kinase inhibitor, had a reducing effect on ALP activity, a differentiation marker in 1,25(OH)(2)D-3-treated primary human osteoblasts. However, SB203580, a potent p38 MAP Kinase inhibitor, had no effect on the differentiation in this system. This indicates that ERK, not p38, is directly related to 1,25(OH)(2)D-3-stimulated ALP activity in primary human osteoblasts. These results also show that the vitamin D-3 analogue stimulates ERK1 activation in primary human osteoblasts. This finding provides one of signaling pathways for differentiation in primary human osteoblasts.

引用

页码：31 / 41

页数：11

共 23 条

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