Binding properties of Echinococcus granulosus fatty acid binding protein

被引:25
作者
Alvite, G
Di Pietro, SM
Santomé, JA
Ehrlich, R
Esteves, A
机构
[1] Fac Ciencias Montevideo, Secc Bioquim, Montevideo 11400, Uruguay
[2] Univ Buenos Aires, Fac Farm & Bioquim, RA-1113 Buenos Aires, DF, Argentina
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2001年 / 1533卷 / 03期
关键词
fatty acid binding protein; binding property; fatty acid; EgFABP1; Echinococcus granulosus;
D O I
10.1016/S1388-1981(01)00164-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
EgFABP1 is a developmentally regulated intracellular fatty acid binding protein characterized in the larval stage of parasitic platyhelminth Echinococcus granulosus. It is structurally related to the heart group of fatty acid binding proteins (H-FABPs). Binding properties and ligand affinity of recombinant EgFABP1 were determined by fluorescence spectroscopy using cis- and trans-parinaric acid. Two binding sites for cis- and trans-parinaric acid were found (K-d(1) 24 +/- 4 nM, K-d(2) 510 +/- 60 nM for cis-parinaric acid and K-d(1) 32 +/- 4 nM, K-d(2) 364 +/- 75 nM for trans-parinaric). A putative third site for both fatty acids is discussed. Binding preferences were determined using displacement assays. Arachidonic and oleic acids presented the highest displacement percentages for EgFABP1. The Echinococcus FABP is the unique member of the H-FABP group able to bind two long chain fatty acid molecules with high affinity. Structure-function relationships and putative roles for EgFABP1 in E. granulosus metabolism are discussed. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:293 / 302
页数:10
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