A contribution to the improvement of Listeria monocytogenes enumeration in cold-smoked salmon

被引:38
作者
Besse, NG
Audinet, N
Beaufort, A
Colin, P
Cornu, M
Lombard, B
机构
[1] Afssa LERHQA, Agence Francaise Secur Sanit Aliments, Afssa Lab Etud & Rech Hyg & Qual Aliments, F-94700 Maisons Alfort, France
[2] Afssa LERAC, Afssa Lab Etud & Rech Alimentat Collect, F-94709 Maisons Alfort, France
[3] Afssa LERPP, Afssa Lab Etud & REch Pathol Poissons, F-29280 Plouzane, France
关键词
Listeria monocytogenes; enumeration; filtration; microbiological methods; cold-smoked salmon;
D O I
10.1016/S0168-1605(03)00375-1
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
For the enumeration of Listeria monocytogenes in food, a sensitive enumeration method based on membrane filtration followed by transfer of the filter to a selective medium has been developed. This study was carried out with cold-smoked salmon, a product likely to be contaminated with L. monocytogenes. The operating protocol utilizes three filtration runs in parallel (5, 15 and 30 ml) of a 1 in 10 dilution of the salmon suspension through 0.45-mum pore-size cellulose ester membranes, and then culture of the filters on Aloa agar (AES Laboratoires, Combourg, France). The results obtained with the technique were compared with those from the reference EN ISO 11290-2 method and found to provide more precise results in the enumeration of L. monocytogenes from both artificially and naturally contaminated cold-smoked salmon. Moreover, for several samples contaminated at low levels, L. monocytogenes could be recovered only by the filtration method. The examination of increasing volumes of salmon suspension enabled readable results to be obtained for all levels of L. monocytogenes and competitive microflora investigated. In most cases, the optimised operating protocol enabled 5.1 g of salmon to be examined, instead of 0.01-0.1 g with the reference EN ISO 11290-2 method, thus improving the sensitivity of the method. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:119 / 127
页数:9
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