Herpes simplex virus type 1-mediated transfer of neurotrophin-3 stimulates survival of chicken auditory sensory neurons

被引：10

作者：

Carnicero, E

Knipper, M

Tan, J

Alonso, MT

Schimmang, T ^{[1
]}

机构：

[1] Univ Valladolid & Consejo Super Invest Cientifica, Inst Biol & Genet Mol, Dept Bioquim Biol Mol & Fisiol, Fac Med, Valladolid 47005, Spain

[2] Univ Tubingen, Dept Otorhinolaryngol, D-72076 Tubingen, Germany

[3] Univ Hamburg, Ctr Mol Neurobiol, D-20251 Hamburg, Germany

来源：

NEUROSCIENCE LETTERS | 2002年 / 321卷 / 03期

关键词：

cochlea; gene transfer; chicken; ototoxicity;

D O I：

10.1016/S0304-3940(01)02501-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neurotrophin-3 (NT-3) is one of the most potent stimulators for survival of auditory sensory neurons. Viral transfer of neurotrophins into auditory neurons may offer a route to provide a permanent supply of the growth factor and guarantee their long-term survival. Herpes simplex virus type 1 (HSV-1)-based vectors have demonstrated their effectiveness to transfer genes into peripheral sensory neurons. In the present report, we have produced a HSV-1 -based amplicon vector expressing NT-3. This vector efficiently infects isolated auditory neurons and stimulates their survival during distinct developmental stages of the inner ear. Therefore, this vector may present a unique entry point to develop therapies preventing or treating hearing impairment caused by the degeneration of auditory neurons. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：149 / 152

页数：4

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