Medaka (Oryzias latipes) FTZ-F1 potentially regulates the transcription of P-450 aromatase in ovarian follicles:: cDNA cloning and functional characterization

被引：92

作者：

Watanabe, M

Tanaka, M

Kobayashi, D

Yoshiura, Y

Oba, Y

Nagahama, Y ^{[1
]}

机构：

[1] Natl Inst Basic Biol, Dept Dev Biol, Reprod Biol Lab, Okazaki, Aichi 4448585, Japan

[2] Grad Univ Adv Studies, Sch Life Sci, Dept Mol Biomech, Okazaki, Aichi 4448585, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1999年 / 149卷 / 1-2期

基金：

日本学术振兴会;

关键词：

cytochrome P-450 aromatase; FTZ-F1-like protein; promoter; transcriptional regulation; fish ovarian follicles; medaka : Oryzias latipes;

D O I：

10.1016/S0303-7207(99)00006-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Our previous findings suggest the activity of cytochrome P-450 aromatase (P-450arom), the enzyme which converts testosterone to estradiol-17 beta, in the ovarian follicle of medaka (Oryzias latipes) is regulated at the transcriptional level. In this study, we cloned a cDNA encoding a FTZ-F1-like protein (mdFtz-F1) from ovarian follicles of medaka. In vitro translated mdFTZ-F1, and nuclear extract from medaka ovarian follicles, formed complexes with oligonucleotide probes containing putative orphan nuclear receptor binding motifs, which are present in the promoter region of the medaka P-450arom gene. The expression pattern of mdFtz-F1 transcripts during oogenesis coincides with that of P-450arom transcripts. Transfection assays further suggest a potential transcriptional regulatory activity of mdFTZ-F1 upon the medaka P-450arom promoter. Taken together, these results suggest a potential role of mdFTZ-F1 in the transcriptional regulation of P-450arom in the ovarian follicle of medaka. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：221 / 228

页数：8

共 32 条

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