Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization

被引：46

作者：

Czlapinski, Jennifer L. ^{[1
]}

Schelle, Michael W. ^{[1
]}

Miller, Lawrence W. ^{[3
]}

Laughlin, Scott T. ^{[1
]}

Kohler, Jennifer J. ^{[1
]}

Cornish, Virginia W. ^{[3
]}

Bertozzi, Carolyn R. ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 40期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1021/ja8037728

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. The most widely used CID, the natural product rapamycin and its relatives, is immunosuppressive due to interactions with endogenous targets and thus has limited utility in vivo. Here we report a new biocompatible CID, Tmp-SLF, which dimerizes E. coli DHFR and FKBP and has no endogenous mammalian targets that would lead to unwanted in vivo side effects. We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. Finally, we engineered the Golgi-resident glycosyltransferase FucT7 for tunable control by Tmp-SLF in mammalian cells.

引用

页码：13186 / 13187

页数：2

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