Inhibition of DNA hybridization by small metal nanoparticles

被引：19

作者：

Yang, J

Lee, JY

Too, HP

Chow, GM

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 119260, Singapore

[2] Natl Univ Singapore, Singapore MIT Alliance, Singapore 117576, Singapore

[3] Natl Univ Singapore, Dept Biochem, Singapore 119260, Singapore

[4] Natl Univ Singapore, Dept Mat Sci & Engn, Singapore 119260, Singapore

来源：

BIOPHYSICAL CHEMISTRY | 2006年 / 120卷 / 02期

关键词：

oligonucleotide; nanoparticle; non-specific interaction; assembly; DNA hybridization;

D O I：

10.1016/j.bpc.2005.10.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inhibition of DNA hybridization by small metal nanoparticles has been examined in detail. DNA melting point analysis showed that the oligonucleotides adsorb strongly and nonspecifically on small metal nanoparticles, inhibiting the hybridization of complementary DNA sequences in common buffered solutions. The nonspecific interaction is even strong enough to disrupt pre-existing hydrogen bonds in short double-stranded DNA. The nonspecific interaction could be weakened by increasing the particle size. As an example, a core-shell assisted method was used to successfully assemble Pt nanoparticles by DNA hybridization that could not be done otherwise. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：87 / 95

页数：9

共 39 条

[1] Organization of 'nanocrystal molecules' using DNA [J].