Large-scale synthesis of H-antigen oligosaccharides by expressing Helicobacter pylori α1,2-fucosyltransferase in metabolically engineered Escherichia coli cells

被引：74

作者：

Drouillard, S ^{[1
]}

Driguez, H ^{[1
]}

Samain, E ^{[1
]}

机构：

[1] CNRS, CERMAV, F-38041 Grenoble 9, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 11期

关键词：

antigens; biosynthesis; gene technology; oligosaccharides; transferases;

D O I：

10.1002/anie.200503427

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Sugar production: A microbiological method has been developed for the production of fucosyl α1,2-linked carbohydrates from lactose. The syntheses of 2′-fucosyllactose and lacto-N- neofucopentaose-1 (see structure), which contain the H-2 antigen (Fucα-2Galβ-4R), were achieved by cultivating Escherichia coli strains that overexpressed the appropriate heterologous genes. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：1778 / 1780

页数：3

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