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Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium leguminosarum biovar trifolii

被引：32
作者：
Paton, GI
Palmer, G
Burton, M
Rattray, EAS
McGrath, SP
Glover, LA
Killham, K
机构：
[1] UNIV ABERDEEN,DEPT MOL & CELL BIOL,ABERDEEN,SCOTLAND
[2] IACR ROTHAMSTED,DEPT SOIL SCI,HARPENDEN,HERTS,ENGLAND
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 24卷 / 04期
关键词：
D O I：
10.1046/j.1472-765X.1997.00071.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied, The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.
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页码：296 / 300
页数：5
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