A fluorimetric and circular dichroism study of hemoglobin - Effect of pH and anionic amphiphiles

被引：76

作者：

De, S ^{[1
]}

Girigoswami, A ^{[1
]}

机构：

[1] Univ Kalyani, Dept Chem, Kalyani 741235, W Bengal, India

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2006年 / 296卷 / 01期

关键词：

D O I：

10.1016/j.jcis.2005.08.047

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this work, bovine hemoglobin (Hb) has been Studied mainly by the fluorescence method. pH has been found to exert a profound effect on Hb structure. This has been confirmed by fluorescence and circular dichroism (CD) studies. The pH-induced change in quaternary structure of Hb indirectly affects its secondary structure. This in turn affects ligand binding to Hb at various pH. The binding of two amphiphiles, a bile salt and a surfactant, have been investigated. The pH-induced structural modification of Hb has been confirmed by studies with the well-known denaturant urea and the polarity probe ANS, which has been used Lis an extrinsic fluorophore. (c) 2005 Published by Elsevier Inc.

引用

页码：324 / 331

页数：8

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