Transglycosylation of cellobiose by partially purified Trichoderma viride cellulase

被引：13

作者：

Kono, H ^{[1
]}

Waelchli, MR

Fujiwara, M

Erata, T

Takai, M

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Sapporo, Hokkaido 0608628, Japan

[2] Bruker Japan Co Ltd, Tsukuba, Ibaraki 3050051, Japan

来源：

CARBOHYDRATE RESEARCH | 1999年 / 319卷 / 1-4期

关键词：

Trichoderma viride cellulase; transglycosylation; oligosaccharide synthesis;

D O I：

10.1016/S0008-6215(99)00105-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A commercial cellulase from Trichoderma viride was fractionated into three fractions, F1, F2, and F3, in order to investigate transglycosylation activities. Among these fi actions, F3, which demonstrated highly hydrolytic activity toward p-nitrophenyl P-D-glucopyranoside and Avicel, most effectively catalyzed the transglycosylation of cellobiose and converted cellobiose into beta-Glc-(1 --> 6)-beta-glc-(1 --> 4)-Glc and beta-Glc-(1 --> 6)-beta-Glc-(1 --> 6)-beta-Glc-(1 --> 4)-Glc. The F3 fraction contained the enzyme to catalyze beta-glucosyl transfer toward only the C-6 position of the sugar acceptor, and thus it is expected to be of use for syntheses of functional oligosaccharides. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：29 / 37

页数：9

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