Life sciences require the third dimension

被引：73

作者：

Keller, PJ ^{[1
]}

Pampaloni, F ^{[1
]}

Stelzer, EHK ^{[1
]}

机构：

[1] EMBL Heidelberg, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany

来源：

CURRENT OPINION IN CELL BIOLOGY | 2006年 / 18卷 / 01期

关键词：

D O I：

10.1016/j.ceb.2005.12.012

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Novel technologies are required for three-dimensional cell biology and biophysics. By three-dimensional we refer to experimental conditions that essentially try to avoid hard and flat surfaces and favour unconstrained sample dynamics. We believe that light-sheet-based microscopes are particularly well suited to studies of sensitive three-dimensional biological systems. The application of such instruments can be illustrated with examples from the biophysics of microtubule dynamics and three-dimensional cell cultures. Our experience leads us to suggest that three-dimensional approaches reveal new aspects of a system and enable experiments to be performed in a more physiological and hence clinically more relevant context.

引用

页码：117 / 124

页数：8

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