Direct enantiomeric separation of N-aminoethylamino acids:: determination of the enantiomeric excess of chiral peptide nucleic acids (PNAs) by GC

被引:31
作者
Corradini, R
Di Silvestro, G
Sforza, S
Palla, G
Dossena, A
Nielsen, PE
Marchelli, R
机构
[1] Univ Parma, Dipartimento Chim Organ & Ind, I-43100 Parma, Italy
[2] Univ Copenhagen, Panum Inst, Ctr Biomol Recognit, DK-2200 Copenhagen, Denmark
关键词
D O I
10.1016/S0957-4166(99)00210-4
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
Direct chiral separation of N-aminoethylamino acids has been performed for the first time by gas chromatographic (GC) analysis with a Chirasil-Val column, after derivatisation with trifluoroacetic anhydride in dichloromethane, which led to the corresponding piperazin-2-one derivatives, as identified by NMR and GC/MS analysis. The method was used for the analysis of the enantiomeric excess of chiral peptide nucleic acid (PNA) monomers and oligomers after hydrolysis with 6N HCl. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2063 / 2066
页数:4
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