Isolation and characterization of inositol sphingophospholipids from Phytophthora parasitica Dastur

被引：10

作者：

Bruneteau, M ^{[1
]}

Fournol, F ^{[1
]}

Gandon, C ^{[1
]}

Becchi, M ^{[1
]}

Pivot, V ^{[1
]}

机构：

[1] CARSO, CNRS, SERV CENT ANAL, F-69390 VERNAISON, FRANCE

来源：

LIPIDS | 1997年 / 32卷 / 04期

关键词：

D O I：

10.1007/s11745-997-0045-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several inositol sphingophospholipids (ISPL) were isolated from mycelia of Phytophthora parasitica Dastur, a phytopathogenic fungus of carnation. The ISPL structures were determined by fast atom bombardment. All ISPL consisted of ceramides linked to inositol phosphate. We investigated the effect of growth conditions on the ISPL produced in four different media that are commonly used for fungal cultures. We showed that P. parasitica Dastur synthesized four major classes of compounds with molecular weights of M-r = 751, 807, 835, and 849 containing the 16:1 base and the 16:0 or 20:0 or 22:0 or 22h:1 N-acyl group. The relative abundance of the different ISPL is dependent on growth conditions.

引用

页码：359 / 362

页数：4

共 29 条

[1] CARBOHYDRATE STRUCTURES OF 3 NOVEL PHOSPHOINOSITOL-CONTAINING SPHINGOLIPIDS FROM THE YEAST HISTOPLASMA-CAPSULATUM [J].

BARR, K ;

LAINE, RA ;

LESTER, RL .

BIOCHEMISTRY, 1984, 23 (23) :5589-5596

[2] TANDEM MASS-SPECTROMETRY OF GLYCOLIPIDS [J].

COSTELLO, CE ;

VATH, JE .

METHODS IN ENZYMOLOGY, 1990, 193 :738-768

[3] STRUCTURE ELUCIDATION OF GLYCOSPHINGOLIPIDS AND GANGLIOSIDES USING HIGH-PERFORMANCE TANDEM MASS-SPECTROMETRY [J].

DOMON, B ;

COSTELLO, CE .

BIOCHEMISTRY, 1988, 27 (05) :1534-1543

[4] ISOLATION AND STRUCTURAL STUDY OF THE GLUCANS OF PHYTOPHTHORA-PARASITICA [J].

FABRE, I ;

BRUNETEAU, M ;

RICCI, P ;

MICHEL, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 142 (01) :99-103

[5]

HALL R, 1969, PHYTOPATHOLOGY, V59, P770

[6] IMPROVED ONE-DIMENSIONAL THIN-LAYER CHROMATOGRAPHIC TECHNIQUE FOR POLAR LIPIDS [J].

HEAPE, AM ;

JUGUELIN, H ;

BOIRON, F ;

CASSAGNE, C .

JOURNAL OF CHROMATOGRAPHY, 1985, 322 (02) :391-395

[7]

HSIEH TCY, 1981, J BIOL CHEM, V256, P7747

[8] STRUCTURE OF A MAJOR GLYCOPHOSPHOCERAMIDE FROM TOBACCO-LEAVES, PSL-I-2-DEOXY-2-ACETAMIDO-D-GLUCOPYRANOSYL (ALPHA-1-]4)-D-GLUCURONOPYRANOSYL (ALPHA-1-]2)MYOINOSITOL-1-O-PHOSPHOCERAMIDE [J].

HSIEH, TCY ;

KAUL, K ;

LAINE, RA ;

LESTER, RL .

BIOCHEMISTRY, 1978, 17 (17) :3575-3581

[9]

HUGUENIN B, 1974, Annales de Phytopathologie, V6, P425

[10]

KANESHIRO ES, 1986, J LIPID RES, V27, P1294

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