Time course of luciferyl adenylate synthesis in the firefly luciferase reaction

被引：15

作者：

Dukhovich, A ^{[1
]}

Sillero, A ^{[1
]}

Sillero, MAG ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC MED,CSIC,INST INVEST BIOMED,DEPT BIOQUIM,E-28029 MADRID,SPAIN

来源：

FEBS LETTERS | 1996年 / 395卷 / 2-3期

关键词：

firefly luciferase; luciferin; ATP; luciferyl adenylate; enzyme kinetics;

D O I：

10.1016/0014-5793(96)01038-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been followed, The properties of luciferyl adenylate, enzymatically or chemically synthesized, as substrate of luciferase, have been compared. The potential use of luciferyl adenylate for luciferase detection is here proposed.

引用

页码：188 / 190

页数：3

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