Hypersaline stress induces the turnover of phosphatidylcholine and results in the synthesis of the renal osmoprotectant glycerophosphocholine in Saccharomyces cerevisiae

被引:26
作者
Kiewietdejonge, A
Pitts, M
Cabuhat, L
Sherman, C
Kladwang, W
Miramontes, G
Floresvillar, J
Chan, J
Ramirez, RM
机构
[1] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA
[2] San Francisco State Univ, Dept Math, San Francisco, CA 94132 USA
[3] Calif Environm Protect Agcy, Reprod & Canc Assessment Sect, Oakland, CA USA
关键词
yeast; phosphatidylcholine turnover; saline stress; glycerophosphocholine; osmoprotectant; transport;
D O I
10.1111/j.1567-1364.2006.00030.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The role of phosphatidylcholine turnover during hypersaline stress is investigated in Saccharomyces cerevisiae. In the wild-type strain, 2180-1A hypersaline stress induced the rapid turnover of phosphatidylcholine, a major membrane lipid. Yeast cells were grown in the presence of [C-14]-choline to label phosphatidylcholine. Upon shifting the cells to medium with 0.8 M NaCl, phosphatidylcholine levels were diminished by c. 30% within 20 min to yield glycerophosphocholine, a methylamine osmoprotectant that has been previously identified in renal cells. High-performance liquid chromatography studies showed that osmotically mediated glycerophosphocholine production was enhanced if 10 mM choline was added as a supplement to synthetic dextrose medium with 1.6 M NaCl, but glycine betaine was not detected. Enhanced glycerophosphocholine production also correlated with improved growth in media containing 1.6 M NaCl and choline. Enhanced growth is specific to methylamines: salt-stressed cells supplemented with 10 mM choline or glycine betaine showed enhanced growth relative to unsupplemented control cultures, but other additives had no effect on growth or adversely affected it. Nutritional effects are ruled out because yeast cannot use choline or glycine betaine as carbon or nitrogen sources in normal or high-salt medium. Finally, enhanced growth in hypersaline media with choline or glycine betaine is dependent on the choline permease Hnm1. These results in yeast highlight a similarity with mammalian renal cells, namely that phosphatidylcholine turnover contributes to osmotic adaptation via synthesis of the osmoprotectant glycerophosphocholine.
引用
收藏
页码:205 / 217
页数:13
相关论文
共 80 条
[1]   CHARACTERIZATION OF THE OSMOTIC-STRESS RESPONSE IN SACCHAROMYCES-CEREVISIAE - OSMOTIC-STRESS AND GLUCOSE REPRESSION REGULATE GLYCEROL-3-PHOSPHATE DEHYDROGENASE INDEPENDENTLY [J].
ALBERTYN, J ;
HOHMANN, S ;
PRIOR, BA .
CURRENT GENETICS, 1994, 25 (01) :12-18
[2]   GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY [J].
ALBERTYN, J ;
HOHMANN, S ;
THEVELEIN, JM ;
PRIOR, BA .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) :4135-4144
[3]   OSMOREGULATION IN SACCHAROMYCES-CEREVISIAE - STUDIES ON THE OSMOTIC INDUCTION OF GLYCEROL PRODUCTION AND GLYCEROL 3-PHOSPHATE DEHYDROGENASE (NAD+) [J].
ANDRE, L ;
HEMMING, A ;
ADLER, L .
FEBS LETTERS, 1991, 286 (1-2) :13-17
[4]   QUATERNARY AMMONIUM-COMPOUNDS IN THE BIOSPHERE - AN EXAMPLE OF A VERSATILE ADAPTIVE STRATEGY [J].
ANTHONI, U ;
CHRISTOPHERSEN, C ;
HOUGAARD, L ;
NIELSEN, PH .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1991, 99 (01) :1-18
[5]   ROLES OF GLYCEROL AND GLYCEROL-3-PHOSPHATE DEHYDROGENASE (NAD+) IN ACQUIRED OSMOTOLERANCE OF SACCHAROMYCES-CEREVISIAE [J].
BLOMBERG, A ;
ADLER, L .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :1087-1092
[6]   Developmental neuroscience - Choline, a vital amine [J].
Blusztajn, JK .
SCIENCE, 1998, 281 (5378) :794-795
[7]   The two biosynthetic routes leading to phosphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling [J].
Boumann, HA ;
Damen, MJA ;
Versluis, C ;
Heck, AJR ;
de Kruijff, B ;
de Kroon, AIPM .
BIOCHEMISTRY, 2003, 42 (10) :3054-3059
[8]   AN OSMOSENSING SIGNAL TRANSDUCTION PATHWAY IN YEAST [J].
BREWSTER, JL ;
DEVALOIR, T ;
DWYER, ND ;
WINTER, E ;
GUSTIN, MC .
SCIENCE, 1993, 259 (5102) :1760-1763
[9]  
BROWN AD, 1986, FEMS MICROBIOL LETT, V39, P31
[10]   WATER RELATIONS OF SUGAR-TOLERANT YEASTS - ROLE OF INTRACELLULAR POLYOLS [J].
BROWN, AD ;
SIMPSON, JR .
JOURNAL OF GENERAL MICROBIOLOGY, 1972, 72 (OCT) :589-&