Analyzing real-time PCR data by the comparative CT method

被引：21130

作者：

Schmittgen, Thomas D. ^{[1
]}

Livak, Kenneth J. ^{[2
]}

机构：

[1] Ohio State Univ, Coll Pharm, Div Pharmaceut, Columbus, OH 43210 USA

[2] Appl Biosyst Inc, Foster City, CA 94404 USA

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 06期

关键词：

D O I：

10.1038/nprot.2008.73

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene. A widely used method to present relative gene expression is the comparative C-T method also referred to as the 2(-Delta Delta CT) method. This protocol provides an overview of the comparative CT method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.

引用

页码：1101 / 1108

页数：8

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