Expression of SKP1 mRNA and protein in rat brain during postnatal development

被引：7

作者：

UroCoste, E

Fonta, C

Hatey, F

Perret, E

Delisle, MB

Caput, D

Imbert, M

机构：

[1] UNIV TOULOUSE 3,FAC MED RANGUEIL,EHESS,CNRS,UMR 5549,CTR RECH CERVEAU & COGNIT,F-31052 TOULOUSE,FRANCE

[2] CHU RANGUEIL,SERV ANAT & CYTOL PATHOL,F-31403 TOULOUSE 4,FRANCE

[3] INRA,LAB GENET CELLULAIRE,F-31326 CASTANET TOLOSAN,FRANCE

[4] SANOFI RECH,LABEGE INNOPOLE,F-31676 LABEGE,FRANCE

来源：

NEUROREPORT | 1997年 / 8卷 / 07期

关键词：

apoptosis; cell cycle; immunocytochemistry; mRNA expression; neuronal; proteolysis; plasticity; SKP1;

D O I：

10.1097/00001756-199705060-00023

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WE previously isolated the cell cycle element SXP1 as a candidate plasticity gene in the rat visual cortex. Here, we studied the expression and localization of SKP1 mRNA and protein in rat brain. We found a high level of expression for the SKP1 gene in the cortex at different postnatal ages. SKP1 mRNA levels remained stable from P2 (postnatal day 2) to adulthood. SKP1 mRNA expression was also detected in several other brain areas. Skp1p (SKPI protein) immunohistochemistry showed nuclear staining in a large majority of neurones. The pyramidal cells in the hippocampus, as well as cortical cells were stained. The presence of Skp1p in post-mitotic neurones suggests that this protein is involved in processes other than the cell cycle: other target proteins and functions in neurones are currently under investigation.

引用

页码：1675 / 1678

页数：4

共 18 条

[1] SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box [J].