RpaB, Another Response Regulator Operating Circadian Clock-dependent Transcriptional Regulation in Synechococcus elongatus PCC 7942

被引：46

作者：

Hanaoka, Mitsumasa ^{[1
,3
,4
]}

Takai, Naoki ^{[4
,5
]}

Hosokawa, Norimune ^{[6
,7
]}

Fujiwara, Masayuki ^{[2
]}

Akimoto, Yuki ^{[1
,3
]}

Kobori, Nami ^{[1
]}

Iwasaki, Hideo ^{[6
,7
,8
]}

Kondo, Takao ^{[4
]}

Tanaka, Kan ^{[1
,3
,4
,9
]}

机构：

[1] Chiba Univ, Div Appl Biol Chem, Grad Sch Hort, Matsudo, Chiba 2718510, Japan

[2] Chiba Univ, Dept Nanobiol, Grad Sch Adv Integrat Sci, Matsudo, Chiba 2718510, Japan

[3] Univ Tokyo, Inst Mol & Cellular Biosci, Mol Genet Lab, Bunkyo Ku, Tokyo 1130032, Japan

[4] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Kawaguchi, Saitama 3320012, Japan

[5] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

[6] Waseda Univ, Dept Elect Engn & Biol Sci, Tokyo, Japan

[7] Waseda Univ, Ctr Adv Biomed Sci TWIns, Tokyo, Japan

[8] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol PRESTO, Shinjuku Ku, Tokyo 1628480, Japan

[9] Tokyo Inst Technol, Chem Resources Lab, Div Bioresources, Midori Ku, Yokohama, Kanagawa 2268503, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 31期

基金：

日本学术振兴会;

关键词：

KAIC PHOSPHORYLATION; GENE-EXPRESSION; HIGH-LIGHT; ACETYL PHOSPHATE; PROTEIN; FEEDBACK; RHYTHMS; SASA; OSCILLATION; UPSTREAM;

D O I：

10.1074/jbc.M111.338251

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The circadian clock of cyanobacteria is composed of KaiA, KaiB, and KaiC proteins, and the SasA-RpaA two-component system has been implicated in the regulation of one of the output pathways of the clock. In this study, we show that another response regulator that is essential for viability, the RpaA paralog, RpaB, plays a central role in the transcriptional oscillation of clock-regulated genes. In vivo and in vitro analyses revealed that RpaB and not RpaA could specifically bind to the kaiBC promoter, possibly repressing transcription during subjective night. This suggested that binding may be terminated by RpaA to activate gene transcription during subjective day. Moreover, we found that rpoD6 and sigF2, which encode group-2 and group-3 sigma factors forRNApolymerase, respectively, were also targets of the RpaAB system, suggesting that a specific group of sigma factors can propagate genome-wide transcriptional oscillation. Our findings thus reveal a novel mechanism for a circadian output pathway that is mediated by two paralogous response regulators.

引用

页码：26321 / 26327

页数：7

共 30 条

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